Reproduction Abstracts

Introduction: Deleted in azoospermia-like (DAZL ) is an RNA-binding protein essential for germ cell entry into meiosis and later stages of germ cell maturation, and its absence is associated with infertility in vertebrates. Efforts to identify in vivo mRNA targets of DAZL have mainly been restricted to mouse, thus human-specific investigations are required.Materials and methods: RT-qPCR and western blotting were used to profile DAZL isoform expr...

Introduction: DAZL (deleted in azoospermia-like) is an RNA binding protein essential for germ cell entry into meiosis and later stages of germ cell maturation, and its absence is associated with infertility in vertebrates. Efforts to study DAZL and its in vivo RNA targets have been largely limited to mouse, thus human-specific investigations are required.Methods: Human DAZL protein was immunoprecipitated from 17 week ovarian lysate bound RNAs we...